Automated Medium Refreshment for Cells Growth

Circuit Based Automation Project with Raspberry Pi Control

This project aims to automate laboratory tasks such as media refreshment while preventing contamination risks by reducing human contact. We developed an automated system using peristaltic pumps, a Raspberry Pi, stepper drivers, and a flow sensor. The gantry system was created in collaboration with Ph.D. students.

Left: Peristaltic pump setup for automated media delivery. Right: Circuit implementation showing control system integration.

Operation Modes

The machine supports two sophisticated types of operations:

1. Continuous Media Refreshment

  • Maintains a fixed flowrate using PID control
  • Flow rate sensor readings provide feedback
  • Stepper driver adjusts speed based on real-time measurements

2. Fixed Volume Refreshment

  • Operates at a set motor speed
  • Integrates flowrate readings over time
  • Automatically stops when desired volume is reached
Demonstration of the automated media refreshment process in action.

Key Features

  • Automated media refreshment
  • Contamination risk reduction
  • Real-time flow monitoring
  • PID-controlled precision
  • Flexible operation modes
  • User-friendly interface

Technical Implementation

The system integrates various components:

  • Raspberry Pi for central control
  • Peristaltic pumps for fluid handling
  • Flow sensors for precise measurements
  • Stepper drivers for motor control
  • Custom-designed gantry system